數(shù)字圖像技術(shù)在黃瓜氮素營(yíng)養(yǎng)診斷上的應(yīng)用研究
宋述堯,教授,博士生導(dǎo)師。1982年 7月畢業(yè)于吉林農(nóng)業(yè)大學(xué)園藝系,1985年7月獲碩士學(xué)位,后留校任教至今。曾任園藝系副主任、??蒲刑幪庨L(zhǎng),現(xiàn)任園藝學(xué)院院長(zhǎng),蔬菜學(xué)學(xué)科帶頭人。兼任中國(guó)園藝學(xué)會(huì)理事、吉林省農(nóng)學(xué)會(huì)常務(wù)理事、吉林省園藝學(xué)會(huì)副理事長(zhǎng)。主要從事蔬菜學(xué)、設(shè)施園藝的教學(xué)與科研工作。主講研究生與本科生蔬菜栽培學(xué)、高級(jí)蔬菜生理學(xué)、設(shè)施園藝學(xué)等課程。發(fā)表學(xué)術(shù)論文60余篇,主編專著 2部。先后承擔(dān)國(guó)家、省級(jí)科研項(xiàng)目20余項(xiàng),獲省科技進(jìn)步二、三等獎(jiǎng)4項(xiàng)。近年來(lái)先后擔(dān)任吉林省農(nóng)業(yè)跨世紀(jì)學(xué)科學(xué)術(shù)帶頭人 、吉林省跨世紀(jì)學(xué)科學(xué)術(shù)帶頭人 、吉林省跨世紀(jì)農(nóng)業(yè)科技骨干、農(nóng)業(yè)部豐收獎(jiǎng)(吉林)評(píng)審專家、吉林省農(nóng)業(yè)綜合開(kāi)發(fā)專家組專家、吉林省農(nóng)業(yè)科技首批專家顧問(wèn)組專家。3m/ ( ! 宋述堯1 , 王秀峰2(1.吉林農(nóng)業(yè)大學(xué)園藝學(xué)院, 長(zhǎng)春 130118;2.吉林省蔬菜花卉研究所,長(zhǎng)春 130033)K 1:試驗(yàn)設(shè)計(jì)了6種氮素水平處理溫室黃瓜,利用數(shù)碼相機(jī)獲取黃瓜冠層圖像,分析了不同供氮水平下黃瓜冠層圖像參數(shù)與施氮量、土壤無(wú)機(jī)氮(Nmin)和植株氮素營(yíng)養(yǎng)指標(biāo)之間的關(guān)系。結(jié)果表明:黃瓜冠層圖像G/(R+G+B)為診斷黃瓜結(jié)果期氮素營(yíng)養(yǎng)狀況的適宜數(shù)字化參數(shù)。建立了評(píng)價(jià)黃瓜結(jié)果期氮素營(yíng)養(yǎng)豐缺的冠層圖像數(shù)字化指標(biāo) G/(R +G+B)的量值標(biāo)準(zhǔn)和氮肥推薦標(biāo)準(zhǔn)。1oM:黃瓜;氮;數(shù)字圖像;營(yíng)養(yǎng)診斷ms |:TP391.41;S642.2 DS M :A cI|:1000-5684(2008)04-0460-06Diagnosis of N Status of Cucumber Using Digital ImageProcessing TechniqueSONG Shu-yao1, WANG Xiu-feng2(1.College of Horticulture , Jilin Agricultural University , Changchun 130118, China ;2.Institute ofVegetable Science of Jilin Province , Changchun 130033, China)Abstract:The digital pictures of the winter wheat canopy were obtained with a Canon digital camera in2004.The G/(R +G +B)ratio from the cucumber canopy was compared, to the Nmin concentration inthe soil,to the sap nitrate concentration in petiole , to the SPAD readings of leaves,and to the total N con- “: 8v S/1 “(20000202-1)l :2008-06-10吉林農(nóng)業(yè)大學(xué)學(xué)報(bào) 2008,30(4):460 465 http :/ xuebao.jlau.edu.cnJournal of Jilin Agricultural University E-mail :jlndxb vip.sina.comDOI :10.13327 /j .j jlau.2008.04 .038centration.The results show significant inverse linear correlation between the G/(R +G+B)ratio andNmin in soil, sap nitrate concentration in petiole , total N, and SPAD readings at bearing fruit stage.G/(R +G+B)can be considered as an important indictor of N status in cucumber.Optimum N rate attop-dress was determined in growth stages.Key words:cucumber;nitrogen;digital image technique;N status diagnosis! ( V v,6rm, 3 8 g 。Bt v ug L= 1wi 2 ,71g q 10% 1 。V g9F 3', v , H9? B 3 Hq0、/ B“5。( T 、 dT“ 0、 0g 3 +,BW Qg ? 3 p。( 9 40% 50%,yN(g 240 kg/hm2), Mv。V3 (T L、= 3 YTable3.Effect of nitrogen levels on dry weight of fruits,shootsand biomassincucumber kg/hm2 NT LEconomic yield(DW)=Shoot weight(DW) Biomass in shoot(DW)0 2 631a 2 936a 5 567a80 3 250b 3 728ab 6 978b160 3 515bc 4 204bc 7 718bc240 3 779c 4 316c 8 095c320 3 930c 4 404c 8 334c400 3 909c 5 055d 8 967c=5 “g 9FB 6t , 9 3 M=M tv8M, Ml=。VV g() , (=! 3, 2。( ,160,240 kg/hm2 2) (!) , G/(R +G +B)S0.391 0.413 ;400 kg/hm2) 5) , G/(R +G +B)0.413 80 1000.391 0.413 0.391 0.413 70 75V 0.388 0.397 0.429 80 1000.416 0.429 0.415 0.427 60 70V 0.401 0.415 0.442 70 900.426 0.440 0.442 0.440 45 50V 0.417 0.426 0.461 50 800.447 0.459 0.447 0.461 25 30V 0.439 0.448 <0.448 03 ) 3m 3S g y wi, LTK5 S、l vBT 3-6, 9 ,( vT “ -7 8, 10 。' k ! ( V 3SG/(R +G+B)$ ,y N(1 3 3m S。(3 3m s;SG/(R +G +B) SiALM11“,B Jial TB 4 。 s;SG/(R +G +B)r Nmin=cb c SM1K, aT N VS。I N 10 、 . 8 T ,K aRI N 3m G/B ,7 . 5B/(R +G+B)KDS。T 3mS B, V ?T= ;+s1。' k ) ( 、 3 、r Nmin# S$,81=cAb i、= s NSPAD 、= 、 c 4 ZET, N( 3m 3SG/(R +G+B) S, H9wi M g (V4)。7,N 7Z HW,C1“3m) / #! N 5 11-12 , # 3S Sgwi 4-5,10 。' kHq/ 3S Sgwi 1、<W k 。 ID: 1 , r ,f . 8 vv !s J.gg, 2000 , 15(3):65-67. 2 I , ,f .T! k/ J. W j<, 2001(6):36-37. 3 Blackmer T M, Schepers J S, Varel G E.Light reflectance com-pared with other nitrogen stress measurements in corn leaves J.A-gron J, 1994, 6:934-938. 4 Jia Liangliang , Cheng Xinping.Use of digital camera to assess ni-trogen status of winter wheat in the northern China plain J.Jour-nal of Plant Nutrition, 2004, 27(3):441-450. 5 ,f , ,. !#gwi J.S j< S,2002, 35(12):1500-1505. 6 ,.3Q ; 8= S J.S j< S, 2003, 36(7):807-812. 7 , c,f ,. 3ms/ ! J .S 3 j<, 2008, 16(1):145-149. 8 , , ). 3m/ . ! J.S . , 2006, 20(5):257-260. 9 Scharpf P C, Lory J A.Calibrating corn color from aerial pho-tographs to predict sidedress nitrogen need J.Agron J, 2002, 94:397-404. 10 . 3m/ (I N ! D.=: j<v,2004. 11 , , V,. = c 3Q ; 1“ J. 3 , 2006(10):3463-3469. 12 l, , X ,. * 4 | = 3m c J .T, 2007, 33(10):1662-1666.465宋述堯等:數(shù)字圖像技術(shù)在黃瓜氮素營(yíng)養(yǎng)診斷上的應(yīng)用研究吉林農(nóng)業(yè)大學(xué)學(xué)報(bào) Journal of Jilin Agricultural University