µ 2 jÛ1 x ë +2é2 ®2ïC¿1,2* (1.µ 2vÐùî 3ý H Üsýµ 2 É ì 410125 2.µ 2 8 vùî îµ 2 É ì 410125) 摘 要 æ¨ Illumina MiSeq 2500©½ Ü û 2ñ 1“ (SJ113a06g19111) 1ñ¹Z ÖÕ (b Ö )Õ± -ªô=r ¥± 3þÉ©½ i²M1 3þÐsr % ) 16S rRNAy V3+V4 u× ) ITS1u×¥¶a“· #± 3þ ²b²TV ü Õ± -ªr (M ) Ê ÖÆ ) ʹª % )0 5 ) Êa0 ) ʤ ) ʹª ) Õ± -ªr Ï¥% )Õ Ë )÷F¶“Äñ÷Ú Õ± ªr % )a )¥M# ²û? 3 MÄr % ) AceaChao Shannon· ûµ î9F9sY¹ 25.26%25.49%a24.51%24.87% 8.28%9.55%7 )¥ Shannon · µñ® Simpson· 9v9 78.45%275.69% Õ±ªr Ï% ) )¥ Ts Ëí (OTU) Ë9µ î¿M% ) OTU ñ Õ± - üA 6Ú9ÿ 28.73%r Ï+µ¥ OTU Ë üAh ) OTUñ MÄ# ËMÄ1% )vhð )¥Mv9Fb 關(guān) 鍵 詞 ô=r ± 3þ“ 中圖分類號 S641.3； Q938.1+3 文獻(xiàn)標(biāo)志碼 ： A 文章編號 ： 10071032(2018)02015106 Effects of pepper cultivation on the microbial diversity of rhizosphere soil KANG Linyu1 LIU Zhoubin1 OU Lijun2 TONG Hui2 PENG Ying2 YUAN Zuhua1,2* (1.Longping Branch, Graduate School of Hunan University, Changsha, Hunan 410125, China; 2.Vegetable Institution, Hunan Academy of Agricultural Science, Changsha, Hunan 410125, China) Abstract: Two pepper inbred lines, SJ113, 06g19111, and a local variety Zhangshu gang were used as materials, also using the Illumina MiSeq 2500 sequencing platform to sequencing the 16S rRNA gene V3 + V4 regions of bacteria and ITS1 region of fungal, in the rhizosphere soil before and after pepper planting. And combining the bioinformatics to analyze the richness, diversity index and community structure of the soil microorganism. The results showed that both Proteobacteria and Acidobacteria were the dominant bacteria in the soil before and after pepper cultivation. Ascomycota, Basidiomycotina and Fusarium were the dominant fungi. The bacteria species in the soil before and after the planting were more abundant and more diversified than the fungi. The relative abundance and community structure of soil bacteria and fungi had changed after pepper planting. The Ace, Chao and Shannon index of soil bacteria was increased,25.26% 25.49%, 24.51% 24.87% and 8.28% 24.87% respectively after pepper planting. The Simpson index of fungi was increased by 78.45% 78.45%, also Shannon index had a different degree of reduce after pepper planting. The number and types of OTU in soil were also changed after pepper planting, the specific OTU type in soil was reduced, while the number of bacteria OTU was significantly increased 28.73% after pepper planting. The effect of variety difference on OTU type of fungi was greater than that of bacteria, and the relative abundance of pathogenic fungi was significantly increased. Keywords: pepper; rhizosphere soil; microbial diversity 收稿日期 20170623 修回日期 20170913 基金項目 SE S/¦ê9à “ (2014BAD05B04) 作者簡介 B Ö (1993i) oµmË ¦ « Vùî 3ö1V Y± 3þaº8бþºTùî 18874923457163.com *YTïC¿ùîôö1V Y v ¡ 3 Ø 3Ä#s0 3þÐùî csyzh888126.com 152 µ 2 jØr V “% ) ”_ “ ) ”r Äb' kÏ Õ± -ªr Ï% ) AceaChao Shannon · (A÷Ú¿ )¥· 7 Simpson · 5A÷®¿ )bV ü Õ± -ªr Ï% )þÕ )÷F¶“Äñ÷Ú ¿r B¥ “% ) ”r b H Õ±ª% )¶A÷ 6ÚþÕ¥“9µ î9F7 )¶í üAMÄ¥ f/ þÕ¥“µ î®b Ð CARTWRIGHT©13 FIERER ©14ª¹r ± 3þ“бþ “ïM1¥²TBÁb/îÕMÄ¥ðy V ? ®¿Õ± -r í±$­ÂÕ± ª ¥­Â± 3þ Á 3 Yb ' kÏr % ) )¥ OTU “Ð Ace Chao · MÄ? p'BÁ% )¥ OTU Ë iµs% ) ) µ¥OTU Ë Ù 9 OTU Ë¥vsÕ±-r Ïcµ +µ¥ OTU Ë7Õ± ªr +µ¥ OTU Ë üAh O ÖÕW ) OTU Ë¥µs v¿% )bV ü Õ±ªr ϱ 3þ¥ Ë Fî? 3 üA¿MbÕMÄ V ? ÖÕ 3É?âVñÏÁ 3¥ô“s ßþr ± 3þ¥YÁ¥15169V ? r ± 3þYV¿Mô“s ßþ¥îs W¤ eÅô= ð± 3þ¥Õ Ë 1718bÕ±ªô“s ßþ¥µsÁr ) W¥M Ü$u å/î ñ +ñ ¥ )v4Ú1920b' kÏ Õ± ª ¾ ) aö¹ ) ´ ) ©±þhð )¥v9Fb - ¦¥ùî219V ü ¾ ) ö¹ ) )h¥ª hð )7ª hð ) 8v 9öø×KÅ îÕ±þ¥ 3É?âÁ±éhÁ .À µ§ yNÕ± Ù ö Pr »û% )þÕ÷F¶¥ “% ) ”r Hö/î )þÕ“h Ov9F±þhð )¥Mb 參考文獻(xiàn)： 1 ¬k Ù Wy©Z 2Pór ± 3þÐr y0¥1“ J £TþÐ 2010 31(4)530535. 2 f w =ç æä©Ûr m 3¦ u±$ Ër ± 3þ+ J¨ 3 ÿÐ 201021(1) 165173. 3 â W£ Ù 3©Û ØÝ|Äñ ¦ýOÆ r ½a!s± 3þM1ùî JÏS 3 ÿ j ÍS;¦ Å §© ZE ¡:ª 3 Ø#ûrT¥Y J J Ö SÐ 2013 34(14)346350 12 >¿ ¡:ûFý/ ùî DÁ¿ =SvÐ 2008 13 ï æ(â Ï ýþ 71o/ ùî DsÜ j vÐ 2015 14 Ú :f +® ¾©¹ Ï4² 7Ï¥¨ùî J 7ÐFý 2010 10(3) 3032 15 Ú :ì¹ Üë½ Íf¶¦ Å §© Z T ¡:þ# O ùÉ ÖÉ¥Y J J Öý¿çÓ ´©ûÑ ¡:îs# FÄ¥Y J a1vÐÐ 2011 32(2)172175 22 ñ£ðŵ v¥þ Ø 7/ ùî#¨DsÜ j vÐ 2013 23 Ùr¦¶ 6£r B© Ï £ç Ï §Tû ÖÉ¥Y J J Ö SÐ 2012 33(16) 314317 24 ñ£ð ¡© bç Ïûѵsâ ÖÉ¥Y JLÜ jk Ö· W j©ç ÏZ TÐûÑ ÖÉMÄ¥Y J 7ùî 2005(4) 3234 26 à ÃbóÚ§©BWç Ï ¡:û ÖÉ¥Y J öê j Û . õT ¡r ± 3þ“¥Y J.ÏS 3 ÿ j<Ð 2015 23(2) 225232. 13 CARTWRIGHT J DZANTOR E K MOMEN B Soil microbial community profiles and functional diversity in limestone cedar gladesJ Catena 2016 147 216224. 14 FIERER N JACKSON R B. The diversity and biogeography of soil bacterial communitiesJ. Proceedings of the National Academy of Sciences of the United States of America2006 103(3) 626631. 15 ¿uM óS Ü©L 3⨠ô“s ßþ¥Fss J j<Ð 2012 21(8)175181. 16 BRENNAN E B ACOSTAMARTINEZ V Cover cropping frequency is the main driver of soil microbial changes during six years of organic vegetable productionJ. Soil Biology and Biochemistry 2017 109188204. 17 MARSCHNER P CROWLEY D E HIGASHI R M Root exudation and physiological status of a rootcolonizing fluorescent pseudomonad in mycorrhizal and non mycorrhizal pepper (Capsicum annuum L.)J Plant and Soil1997 189(1) 1120. 18 ñ _ ö Ó¦ô“s ßþº/±þ r± 3þºT1“ùîÉZÐZ¬ J±þ 3 ÿÐ2014 38(3) 298310. 19 CHAPARRO J M BADRI D V VIVANCO J M. Rhizosphere microbiome assemblage is affected by plant developmentJ The ISME Journal 2014 8(4) 790803. 20 Zï d1SÜ© Kõô=± 3þ ²# î ÿMÄ¥ùî Jr Ð 2005 42(3)488494. 21 ¥qîÛf¦É©õ¤Õ0zhð )¥s ÖÐkç JÏSõ¤ SÐ 2014(5) 8488 責(zé)任編輯：毛友純 英文編輯：雷東陽